Figure 6.

Effects of sqn-6, miR156 target-site mimic, and overexpression of miR156 on PP TC development. A, PP TC development in leaf 5 of 30-d-old plants was reduced in sqn-6 and 35S::MIM156 (target-site mimic) lines compared to Col-0, whereas it was increased in leaf 10 of 35-d-old plants in 35S::MIR156a (overexpression) relative to Col-0. Effects on PP TC development were more significant in the basal-third compared to the apical-third of leaves. B, RT-qPCR analysis of transcripts isolated from leaves at the same developmental stages as leaves subjected to mPS-PI staining and PP TC analysis as shown in A. Relative abundance was set to 1 for leaf 5 of Col-0 for all transcripts. Data are mean ± se across at least four biological replicates.