Effects of overexpression of SPL3, SPL9, SPL10, and SPL15, and of spl9-4, spl15-1, and spl9-4/spl15-1 mutants, on PP TC development. A, PP TC development was reduced in leaf 5 of 30-d-old rSPL9, rSPL10, and SPL15-1D plants but was not significantly affected in rSPL3 compared to Col-0 (P < 0.01, Student’s t test). B, spl9-4 and spl15-1 single mutants did not show significant difference from Col-0 levels, whereas the spl9-4/spl15-1 double mutant showed a significant increase in PP TC development compared to Col-0 (P < 0.01, Student’s t test). Data are mean ± se across three or four biological replicates.