Fig. 2.

Ceramide and sphingosine levels in the livers and spleens of Farber disease mice after a single injection of rhAC. Groups of ~ 9-week-old Farber disease mice received single i.p. injections of purified rhAC at the indicated doses (n = 3/dose). After 24 h the mice were euthanized and the amounts of total ceramide (Cer) and sphingosine (Sph) were quantified and expressed per milligram total protein in tissue extracts. “0” indicates age-matched Farber mice that were injected with PBS. *indicates p value < 0.05 comparing mice injected with rhAC to mice injected with PBS; **indicates p value < 0.01.