Fig. 10.

Effect of rhAC on chondrocytes from Farber disease mice. Primary chondrocytes were obtained from the humeri of 4-6 week-old Farber disease mice. Cells from 5 to 10 mice were pooled to establish cultures, and grown in standard media (−) or media containing purified rhAC (12.5 μg/ml) for 7 days or 14 days. rhAC was added once at the time of cell plating. qPCR was then used to assess the expression of three chondrogenic marker genes: collagen 2, aggrecan and Sox-9. The dotted lines indicate the expression of these genes in chondrocytes grown from healthy mice in standard media. The results of two separate experiments (Exp 1 and 2) are shown.