Relative number of infected astrocytes and neurons/section during development. The number of immunoreactive astrocytes (blue) and neurons (red) was counted at 4, 5, 7, and 10 dpi (n = 3/time point). Bar indicates SD. Initially at 4 and 5 dpi, most of the cells had the morphology of astrocytes in all 4 areas studied. By 7 dpi, astrocytes were still more numerous than neurons in thalamus and hypothalamus, whereas neurons were more numerous in cortex and hippocampus. By 10 dpi, infected neurons were more prevalent in all areas studied.