Figure 9.

Infection of visual system and other brain loci after intraperitoneal inoculation. A–C, Retina at 4 dpi after P0 inoculation; intraperitoneal ZIKV infects the ganglion cell layer (GCL) and the inner nuclear layer (INL). Immunoreactive processes are found in the internal plexiform layer (IPL). Red represents ZIKV immunoreactivity. Blue represents DAPI counterstain. Scale bar, 8 μm. D, ZIKV in superior colliculus. E, ZIKV in optic chiasm (OC). F, Directly caudal to the optic chiasm is the median eminence (ME), which also showed infection. Scale bar, 15 μm. G, Transgenic mouse expressing GFP in retinal POMC cells was inoculated at P0. By 7 dpi, both GFP-expressing amacrine cells (double arrowhead) and GFP-negative cells showed ZIKV infection. Green represents POMC amacrine cells. Orange represents ZIKV. Scale bar, 15 μm.