Fig 1. ACY-241 is a selective HDAC6 inhibitor that inhibits myeloma cell proliferation and survival.

A) MM.1s cells were treated with ACY-241 for 18 hrs and lysates western blotted for Ac-α-Tubulin, Ac-Histone H3K9, total α-Tubulin and total histone H3. B) Blood from healthy donors and MM patients were treated for 18 hrs ex vivo with ACY-241 and stained with αCD3 and either Ac-α-tubulin or Ac-Histone H2BK5 along with isotype controls. CD3⁺ cells were gated followed by measuring MFI and normalizing against isotype and DMSO treated samples. C) H929 cells were treated with ACY-241 and live cells were counted following trypan blue staining. D) MM cell lines were treated with increasing concentrations of ACY-241 for 4 days and cell viability was measured using an MTS assay. The IC₅₀ was determined for each cell line.