Fig 4. Cells subcultured from melanoma and seeded in Matrigel undergo cell-aggregate and aggregate-aggregate coalescence.

The three examples in panels A, B and C were reconstructed at different times and different locations of a single preparation. A. Example of a cell (blue) translocating to a small aggregate (red) and coalescing with it, in a four hour period. B. Example of three individual cells (green, yellow, blue) coalescing with an aggregate (red), between 45.5 and 49 hours. C. Examples of cell-aggregate and aggregate-aggregate coalescence between 140 and 279 hours of reconstruction. In these panels, the original cells and small aggregates that undergo coalescence are multicolored and numbered so that they can be monitored through coalescence. Note that cells and aggregates 1, 2, 3, 7, 8, 9, 10, 11 and 12 form the aggregate labeled 1/2/3/7/8/9/10/11/12 and cells 4, 5 and 6 form 4/5/6 aggregate.