Fig 5. Tracking of a single cell to an aggregate and subsequent coalescence.

The preparation was that of a secondary culture of melanoma cells derived from a fresh melanoma fragment. A. 3D reconstructions of a single cell (yellow, C1) in the neighborhood of two aggregates (blue, A1; grey A2) that extends three processes, translocates in the direction of the extension directed towards the blue aggregate, A1, and coalesces with it. B. Centroid (center of mass) tracks of the cell C1 and the aggregates A1 and A2, all reconstructed in panel A, between 224 and 241 hours, reveal directed translocating of the cell towards aggregate A1 prior t o coalescence.