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H3K27ac, FOXA1, AR, HOXB13, GATA2 ChIP experiments were performed in LNCaP cells and followed by ChIP–qPCR analysis of H3K27ac (A) and chromatin occupancy of TFs on 5 selected target regions including rs6057110 (B) rs2742624 (C) rs2659051 (D) rs10936845 (E) and rs9925556 (F). And KLK3 enhancer locus was tested as a positive control for ChIP-qPCRs.
