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. 2016 Jul 9;7(34):54616–54626. doi: 10.18632/oncotarget.10520

Figure 4. CRISPR/cas9 -mediated genomic deletion of rs2742624 locus diminishes UPK3A gene expression.

Figure 4

(A) The scheme for deletion of rs2742624-containing enhancer by CRISPR/cas9 technology. The guide RNAs were shown in red and protospacer adjacent motifs(PAM) were underlined. (B–C). PCR genotyping (C) and Sanger sequencing (B) to confirm the biallelic deletion of the rs2742624 locus (168bp) in the positive clone. (D) The expression of UPK3A gene was diminished upon deletion of the rs2742624 locus by RT-qPCR assay, indicating that UPK3A gene is a direct target of the polymorphic enhancer.