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Infectivity of the RC.V8.NLS viral mutant. QT6 cells were transfected with either the wild-type, RC.V8.NLS, or RC.V8.KR/AA proviral constructs and passaged in culture every 3 days for 21 days. Medium was collected prior to each passage, pelleted through a 25% sucrose cushion, and frozen. At the end of the 3-week period, RT assays on all of the stored samples were performed in triplicate and average values were determined for each.
