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. 2017 Feb 15;6:e23282. doi: 10.7554/eLife.23282

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© 2017, Kieffer et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 1. — (A) Mean blood p24 levels from three HIV-1–infected PBMC-NSG mice assessed at 0, 12, and 21 days PI. Error bars represent standard deviation. (B) IF of GALT from HIV-infected animal with highest blood p24 level from panel A at 21 days PI. Samples were stained for human CD3 (magenta), HIV-1 p24 (green), and nuclei (blue). White arrows indicate human CD3+/p24+ cells. (C) Montaged projection EM overview of colon crypt region from the same animal. Areas with virus are highlighted (green asterisks) along with a region of interest for imaging by ET (yellow box). Virus was found in close proximity to a blood vessel (BV) containing red blood cells (RBCs). (D) Tomographic slice of region of interest from panel C showing separate pools of free virus on two sides of an infected cell. Black arrows indicate budding virions.

DOI: http://dx.doi.org/10.7554/eLife.23282.002

Figure 1—source data 1. Source data for Figure 1A.
DOI: http://dx.doi.org/10.7554/eLife.23282.003

elife-23282-fig1-data1.xlsx^{(35.7KB, xlsx)}

DOI: 10.7554/eLife.23282.003