Figure 1. Identification of Nup98-interacting proteins.
Plasmids encoding GFP-Nup98 or GFP alone were transfected into HEK293T cells. These proteins were immunoprecipitated from whole cell lysates using an antibody directed against GFP. Co-immunoprecipitated proteins were analyzed by SDS-PAGE and gel pieces containing regions of interest were analyzed by LC-MS/MS to identify proteins co-immunoprecipitated with GFP-Nup98. Western blotting of these fractions using anti-GFP antibodies confirmed the presence of GFP and GFP-Nup98 (bottom panel). Protein species indicated on the right of the gel represent those producing peptides most frequently identified by LC-MS/MS in the GFP-Nup98 immunoprecipitated fractions. The positions of molecular mass markers (shown in kDa) are indicated on the left.