Figure 12. Nup98 stimulates the transcriptional activity of DHX9.
HEK293T cells transfected with the luciferase gene under control of a cAMP-regulatory element (CRE) were co-transfected with two plasmids, one containing GFP-NUP98 or GFP and another containing either DHX9WT, the point mutant DHX9I347A, the point mutant DHX9K417R or an empty plasmid. Luciferase activity is shown on the y-axis. The luciferase activity from cells transfected with luciferase plasmid alone was designated 1. Each value of relative luciferase activity represents the mean ± standard deviation (n = 3). (** indicate p-value < 0.01 in T-test comparing normalized luciferase activity in cells transfected with GFP-Nup98 versus GFP). DHX9 and the DHX9 point mutants are expressed at similar levels (Figure 12—figure supplement 1).
Figure 12—figure supplement 1. DHX9 point mutant constructs are expressed at levels similar to WT.
Western blots of proteins derived from HEK293T cell lysates expressing the indicated DHX9 constructs were performed. These constructs contain a C-terminal HA-tag, allowing detection and comparison of protein levels. The positions of molecular mass markers (shown in kDa) are indicated on the left. α-tubulin was used as loading control.