FIG. 2.

Inhibition of classical PKC isozymes impairs RSV infection and reduces virus production. (A) Confluent NHBE cells were treated with the myristoylated PKC-α/β pseudosubstrate peptide at the indicated concentrations for 30 min before being infected with RSV at an infectious dose of 1 MOI. The infection was allowed to proceed for 16 h. Infected cells were detected by single-cell immunofluorescence assays (magnification, ×200). (B) NHBE cells grown in a 12-well plate configuration were incubated with different concentrations of the myr-PKC peptide 30 min prior to being infected with 0.1 MOI of rgRSV. Infection was allowed to proceed for 60 h. Culture medium from each treatment condition was collected, and the viral titer was measured. The results are the averages ± standard deviations of three different experiments per experimental condition.