FIG. 3.

PKC-α/β pseudosubstrate peptide does not interfere with the RSV binding to NHBE cells. Confluent NHBE cells were treated with either the PKC pseudosubstrate peptide (control, nonmyristoylated) or the myristoylated PKC pseudosubstrate peptide (myr-PKC peptide) at the indicated concentrations for 30 min before being infected with RSV at an infectious dose of 1 MOI. The infection was allowed to proceed for 16 h. Cell culture monolayers were then detached by trypsin treatment, and single-cell suspensions were processed for analysis by fluorescence-activated cell sorter. The infected cells were detected by an FITC-labeled mouse monoclonal anti-RSV N protein antibody.