FIG. 4.

Myristoylated moiety did not block RSV binding to cells. Confluent NHBE cells were treated with either the myr-autocamtide or the myr-PKC peptide at the indicated concentrations for 30 min before being infected with rgRSV at an infectious dose of 1 MOI. The infection was allowed to proceed for 16 h. Cell culture monolayers were then detached by trypsin treatment, and single-cell suspensions were processed for analysis by fluorescence-activated cell sorter. Infected cells were detected by the fluorescence emitted by enhanced green fluorescent protein produced by rgRSV.