FIG. 5.
Expression of DN-PKC-α reduced the number of RSV-infected cells. Approximately 8 × 105 NHBE cells grown in 6-well plates were transfected with 1 μg of either pDN-PKC-α or pcDNA3.1. Along with these plasmids, 100 ng of pDsRed2N1 was also cotransfected to visualize transfected cells by flow cytometry. Twenty-four hours later, cells were infected with rgRSV, and infection was indicated by the expression of enhanced green fluorescence protein. The percentage of infection in red fluorescent cells (infection in transfected cells) was then determined by flow cytometry.