Figure 2.

miR-204-5p acts as a tumor suppressor in melanoma. (A) Transfection efficiency of the miR-204-5p mimic was determined by PCR. (B) The proliferative ability of A375 cells was measured by a CCK-8 assay. (C and D) Effect of miR-204-5p on apoptosis of A375 cells was assessed by flow cytometry. (E and F) The effect of miR-204-5p on the invasive capacity of melanoma cells was assessed by a Transwell assay. (G and H) The effect of miR-204-5p on the migratory ability of melanoma cells was assessed by the scratch wound assay. *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: PCR, polymerase chain reaction; CCK-8, cell-counting kit-8; NC, negative control; PI, Propidium iodide; OD, optical density.