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. 2017 Feb 27;10:1237–1246. doi: 10.2147/OTT.S128819

Figure 3.

Figure 3

MMP9 and BCL2 are the direct functional targets of miR-204-5p. (A) The binding sites of miR-204-5p within the 3′-UTR of MMP9 and BCL2 were predicted by miRanda. (B) Overexpression of miR-204-5p suppressed luciferase activity in cells with the WT plasmid but did not cause a significant change in A375 cells with the Mut plasmid. (C) Western blots identified MMP9 and BCL2 protein expression changes following transfection with a miR-204-5p mimic or NC; GAPDH was used as a control. (D) PCR to assess the mRNA level of MMP9 and BCL2 in A375 cells following transfection with a miR-204-5p mimic. (E) The heat map shows that MMP9 and BCL2 are negatively correlated with miR-204-5p in thirty malignant melanoma tissues. (F) The Pearson correlation of miR-204-5p and MMP9 and BCL2 expression in thirty malignant melanoma tissues. **P<0.01.

Abbreviations: MMP9, matrix metalloproteinases-9; BCL2, B-cell lymphoma-2; Mut, mutant type; PCR, polymerase chain reaction; UTR, untranslated region; WT, wild type; NC, negative control; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.