Figure 4.

Expression of MMP9 and BCL2 attenuates the effects of miR-204-5p in melanoma. (A) Western blots identified MMP9 and BCL2 protein expression changes following transfection with miR-204-5p alone or in combination with MMP9 and BCL2. (B) BCL2 plasmid reversed the effect of miR-204-5p on the proliferative ability of A375 cells. (C and D) Expression of BCL2 reversed miR-204-5p-induced apoptosis as assessed by flow cytometry. (E and F) The effect of miR-204-5p on the invasive ability of A375 cells was largely abrogated by the MMP9 plasmid. (G and H) The MMP9 plasmid reversed the effect of miR-204-5p on the migratory ability of A375 cells. *P<0.05, **P<0.01.

Abbreviations: MMP9, matrix metalloproteinases-9; BCL2, B-cell lymphoma-2; NC, negative control; PI, Propidium iodide; OD, optical density.