Figure 2.

HOTAIRM1 is involved in DC differentiation.

Notes: (A) The HOTAIRM1 RNA concentration was analyzed by RT-PCR in the process of monocyte differentiation to DCs. (B) HOTAIRM1 lncRNA level in monocytes stimulated with LPS or TGF-β. Flow cytometry to detect CD14 (C) and B7H2 (D) in cells transfected with HOTAIRM1 or NC siRNA. Each experiment was independently performed three times. *P<0.05; **P<0.01.

Abbreviations: DCs, dendritic cells; lncRNA, long noncoding RNA; RT-PCR, real-time polymerase chain reaction; NC, negative control.