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. 2004 Dec;78(24):13966–13974. doi: 10.1128/JVI.78.24.13966-13974.2004

FIG. 4.

FIG. 4.

(A) Symptoms in N. benthamiana plants induced by pBinPLUS-A-1.7 coinoculated with different βC1 mutants. From left to right, plants were coinoculated with pBinPLUS-2β, pBinPLUS-C1M-Fβ, pBinPLUS-C1M-Sβ, pBinPLUS-C1M-Bβ, and pBinPLUS-C1M-Tβ, respectively. The wild-type DNAβ induced severe downward leaf curling, stunting, vein swelling and darkening, and enations. When the first ATG of the βC1 gene was mutated, plants developed leaf curl symptoms but did not become stunted. Plants inoculated with any of the other βC1 mutants remained symptomless. (B) Southern blot analysis of plants inoculated with βC1 mutants of TYLCCNV-Y10 DNAβ. Samples (10 μg) of total nucleic acids were extracted from individual N. benthamiana plants infected by agroinoculation with TYLCCNV-Y10 (lanes 1 and 2), TYLCCNV-Y10 and DNAβ (lanes 3 and 4), TYLCCNV-Y10 and DNAβ-C1MF (lanes 5 and 6), TYLCCNV-Y10 and DNAβ-C1MS (lanes 7 and 8), TYLCCNV-Y10 and DNAβ-C1MB (lanes 9 and 10), and TYLCCNV-Y10 and DNAβ-C1MT (lanes 11 and 12). The blots were probed with the full-length sequence of TYLCCNV-Y10 DNAβ. ssDNA, single-stranded DNA; scDNA, supercoiled DNA.