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(A) The translational levels of invasive, autophagy, and EMT-related markers were determined by western blotting; (B) cells were stained with both Annexin V-APC and PI, and cells that were positive for Annexin V-APC were counted as apoptotic cells; (C) the proportion of apoptotic cells for ECA109 group, control group, and ARHI group, **P<0.01.
Abbreviations: ARHI, aplysia ras homolog I; EMT, epithelial–mesenchymal transition; APC, allophycocyanin; PI, propidium iodide.
