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. 2017 Feb 27;10:1227–1235. doi: 10.2147/OTT.S125185

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© 2017 Tong et al. This work is published and licensed by Dove Medical Press Limited

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WWOX mRNA expression level was positively correlated with the RP11-190D6.2 lncRNA overexpression or knockdown in EOC cell lines.

Notes: Real-time PCR was performed to evaluate the expression levels of RP11-190D6.2 and WWOX, aiming to investigate their potential association. (A) The RP11-190D6.2 expression was positively correlated with WWOX expression (R=0.45519, P<0.00777). (B) The expression of RP11-190D6.2 was significantly lower in each EOC cell line compared with HOSE cell line. Additionally, the expression of RP11-190D6.2 was the highest in HEY-A8, but the lowest in H08910-PM. (C) The data showed that the lncRNA RP11-190D6.2 expression significantly increased. Meanwhile, using real-time PCR, we found that the mRNA expression level of WWOX was apparently upregulated by pcDNA RP11-190D62. (D) In RP11-190D6.2 siRNA groups, we found that RP11-190D6.2 expression was evidently decreased, as was WWOX expression. *P<0.05.

Abbreviations: EOC, epithelial ovarian cancer; HOSE, human ovarian surface epithelial; PCR, polymerase chain reaction; WWOX, WW domain-containing oxidoreductase; pcDNA control, pcDNA, plasmid complementary DNA; lncRNA, long noncoding RNA; mRNA, messenger RNA; siRNA, small interfering RNA; NC, negative control.

WWOX mRNA expression level was positively correlated with the RP11-190D6.2 lncRNA overexpression or knockdown in EOC cell lines.

Notes: Real-time PCR was performed to evaluate the expression levels of RP11-190D6.2 and WWOX, aiming to investigate their potential association. (A) The RP11-190D6.2 expression was positively correlated with WWOX expression (R=0.45519, P<0.00777). (B) The expression of RP11-190D6.2 was significantly lower in each EOC cell line compared with HOSE cell line. Additionally, the expression of RP11-190D6.2 was the highest in HEY-A8, but the lowest in H08910-PM. (C) The data showed that the lncRNA RP11-190D6.2 expression significantly increased. Meanwhile, using real-time PCR, we found that the mRNA expression level of WWOX was apparently upregulated by pcDNA RP11-190D62. (D) In RP11-190D6.2 siRNA groups, we found that RP11-190D6.2 expression was evidently decreased, as was WWOX expression. *P<0.05.

Abbreviations: EOC, epithelial ovarian cancer; HOSE, human ovarian surface epithelial; PCR, polymerase chain reaction; WWOX, WW domain-containing oxidoreductase; pcDNA control, pcDNA, plasmid complementary DNA; lncRNA, long noncoding RNA; mRNA, messenger RNA; siRNA, small interfering RNA; NC, negative control.