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Flowchart of SILAC coupled with LC–MS/MS for a comparative analysis of protein expression in A549 cells treated with PTX and Abr for 24 h.
Abbreviations: 1D, one dimensionally; Abr, Abraxane; Arg, arginine; LC–MS, liquid chromatography–tandem mass spectrometry; Lys, lysine; MS, mass spectrometry; PAGE, polyacrylamide gel electrophoresis; PTX, paclitaxel; SDS, sodium dodecyl sulfate; SILAC, stable isotope labeling with amino acids in cell culture.
