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. 2017 Feb 28;12:1621–1637. doi: 10.2147/IJN.S124403

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© 2017 Ng et al. This work is published and licensed by Dove Medical Press Limited

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ZnO NP treatment causes a significant induction of intracellular ROS in MRC5 cells.

Notes: (A) ROS generation induced by 50 μg/mL ZnO NPs treatment as measured by FACS. There was an increase in DCFDA fluorescence intensity as indicated by a right shift in the treated cells as compared to the control cells. (B) Real time PCR analysis shows an increasing trend in the expression of ER stress-related genes in MRC5 lung fibroblasts following 16 h treatment at 25 and 50 μg/mL of ZnO NPs. Error bars = SEM; *P<0.05; ***P<0.001.

Abbreviations: DCFDA, 2′,7′-dichlorofluorescin diacetate; ER, endoplasmic reticulum; FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; NPs, nanoparticles; PCR, polymerase chain reaction; ROS, reactive oxygen species; SEM, standard error of the mean.