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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Neurochem. 2017 Feb 16;140(6):941–954. doi: 10.1111/jnc.13957

Figure 2.

Figure 2

HNE increases LC3 and p62 protein levels in neurons. (A – C) Cortical neurons were treated with 0.05% ethanol (Con) or 5 µM HNE with or without 100 µM bafilomycin A1 for the indicated time periods. Immunoblots of neuronal cell lysates were analyzed using antibodies against LC3I/II and p62, and β–actin (loading control). Representative blots are shown in panel A, and results of densitometric analysis of the LC3II, p62 and β–actin bands are shown in panels B and C. Values (mean and SEM) are from 4 independent experiments. (D – F) Total cellular lysates from neurons subjected to the indicated individual or combined-treatments (5 µM HNE, 100 nM rapamycin and 100 nM bafilomycin A1) were subjected to immunoblot analysis using antibodies against LC3, p62 and β-actin. Representative blots are shown in panel D, and results of densitometric analysis of the LC3II, p62 and β–actin bands are shown in panels E and F. Values (mean and SEM) are from 4 independent experiments. *p<0.05, **p<0.01, ***p<0.001 compared to the control value.

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