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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Neurochem. 2017 Feb 16;140(6):941–954. doi: 10.1111/jnc.13957

Figure 4.

Figure 4

Neurons exposed to HNE exhibit decreased levels of markers of chaperone-mediated autophagy. (A) Representative immunoblot showing relative levels of LAMP2a and Hsc70 in neurons treated with 0.05% ethanol (Con) for 24 hours or 5 µM HNE for the indicated time periods. (B and C) Results of densitometric analyses of immunoblots from 5 separate experiments. *p<0.05; **p<0.01; ***p<0.001 compared to the control value. (D and E) Levels of Lamp2a (D) and Hsc70 (E) mRNAs were quantified using real-time PCR in RNA samples from neurons treated with ethanol or HNE for the indicated time periods.

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