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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Neurochem. 2017 Feb 16;140(6):941–954. doi: 10.1111/jnc.13957

Figure 5.

Figure 5

Neurons exposed to HNE exhibit accumulations of polyubiquitinated proteins. (A) Representative images showing ubiquitinated protein immunoreactivity in neurons exposed to vehicle (Con) or 5 µM HNE for 6 hours. Scale bar, 10 µm. (B) Puncta of ubiquitinated protein aggregates were quantified in 22 neurons in control cultures and 30 neurons in HNE-treated neurons in 3 separate experiments. ***p<0.001. (C, E, I J and K) Total protein from neurons treated with ethanol or HNE for 6 hours (C, E and G) or 24 hours (I and K) were separated in 4–12% Bis-Tris gels, and the proteins were then transferred onto a nitrocellulose membrane. Representative examples of blots stained with Ponceau S (left) and immunoreactivity with the indicated antibodies (right) are shown. (D, F, H, J and L) Results of densitometric analyses of immunoblots from 3 separate experiments. **p<0.01, ***p<0.001.

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