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. 2017 Feb;13(2):619–630. doi: 10.1016/j.nano.2016.07.008

Figure 4.

Figure 4

In vitro uptake of synthetic AMCP nanoparticles and delivery of cargo into cells: (A) Flow cytometric quantitation of cells that have taken up synthetic calcein-labeled AMCP within the lymphocyte and monocyte gates (n = 3 ± SD). (B) Image Stream analyses of PBMC that were incubated with synthetic calcein-labeled AMCP displaying the monocyte population that have taken up AMCP (n = 4 ± SD). **P = 0.01 versus vehicle. (C). Example of Image Stream dot plot from one donor showing gated CD14+ cells positive for the AMCP label calcein, and (D) graph showing the average percentage of CD14+ cells positive for calcein after 3 h incubation with AMCP particles synthesized with or without Af555 labeled BSA. (E) Example histogram plot of calcein/Af555-BSA similarity scores of double positive cells for 1 subject after 3 h incubation, showing high and low similarity regions. (F) Pie Chart visualizing percentage of CD14+ (calcein/Af555 double positive) cells falling within high (yellow) and low (green) similarity regions: data shown are the average of experiments performed on cells from 3 subjects. (G). Example cell images of calcein/Af555 double positive CD14 cells falling within the high and low similarity gate.