TABLE 5.
IHC detection of PCV2 antigen in lymph nodes, tonsils, and spleens of inoculated and control pigs
| Group | Inoculum | dpi | No. of pigs with PCV2 antigen/no. testeda
|
||
|---|---|---|---|---|---|
| Lymph node | Tonsil | Spleen | |||
| 1 | PBS buffer | 21 | 0/5 (0.0) | 0/5 (0.0) | 0/5 (0.0) |
| 42 | 0/5 (0.0) | 0/5 (0.0) | 0/5 (0.0) | ||
| 2 | PCV2 VP120 | 21 | 0/5 (0.0) | 0/5 (0.0) | 1/5 (0.2) |
| 42 | 2/6 (0.3) | 3/6 (0.5) | 0/6 (0.0) | ||
| 3 | PCV2 VP1 | 21 | 5/5 (1.6) | 4/5 (1.0) | 4/5 (1.2) |
| 42 | 3/5 (0.8) | 2/5 (0.4) | 2/5 (0.4) | ||
a
Values in parentheses are the mean scores of the level of PCV2 antigen in lymphoid tissues (scores ranged from 0 for no antigen detected to 3 for high levels of antigen). The PCV2 antigen in lymph nodes and tonsils at 21 dpi were significantly different (P < 0.05 by Fisher's exact test) in pigs from groups 1, 2, and 3.