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Levels of proteins encoded by E2F-regulated genes are elevated in E6-expressing cells. Total protein extracts (100 μg) from cell populations expressing the different viral proteins indicated were applied to an SDS-10% polyacrylamide gel, transferred onto a PVDF membrane, and incubated with an anti-E2F-1, -CDC2, -cyclin A, or -β-tubulin antibody. The β-tubulin signal was used as a loading control.
