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. 2004 Dec;78(24):14057–14061. doi: 10.1128/JVI.78.24.14057-14061.2004

TABLE 1.

Synthetic oligonucleotides for engineering the full-length SCoV 3C-like protease used in this work

Name Sequence (5′-3′) nta Orientation
S1 TCTGCTGTTCTGCAGAGTGGTTTTAGGAAAATGGCATTCCCGTCAGGCAAAGTTGAAGGG −15-45 Sense
S2 CATTAAGAGTTGTAGTTCCACAGGTTACTTGTACCATGCACCCTTCAACTTTGCCTGACG 26-85 Antisense
S3 TGGAACTACAACTCTTAATGGATTGTGGTTGGATGACACAGTATACTGTCCAAGACATGT 66-125 Sense
S4 TTCATAGTTAGGATTAAGCATGTCTTCTGCTGTGCAAATGACATGTCTTGGACAGTATAC 106-165 Antisense
S5 TGCTTAATCCTAACTATGAAGATCTGCTCATTCGCAAATCCAACCATAGCTTTCTTGTTC 146-205 Sense
S6 ATAGAATGGCCAATAACACGAAGTTGAACATTGCCAGCCTGAACAAGAAAGCTATGGTTG 186-245 Antisense
S7 CGTGTTATTGGCCATTCTATGCAAAATTGTCTGCTTAGGCTTAAAGTTGATACTTCTAAC 226-285 Sense
S8 CAGGTTGGATACGGACAAATTTATACTTGGGTGTCTTAGGGTTAGAAGTATCAACTTTAA 266-325 Antisense
S9 ATTTGTCCGTATCCAACCTGGTCAAACATTTTCAGTTCTAGCATGCTACAATGGTTCACC 306-365 Sense
S10 GGTATGATTAGGTCTCATGGCACACTGATAAACACCAGATGGTGAACCATTGTAGCATGC 346-405 Antisense
S11 CCATGAGACCTAATCATACCATTAAAGGTTCTTTCCTTAATGGATCATGTGGTAGTGTTG 386-445 Sense
S12 ATATAGCAGAAAGACACGCAATCATAATCAATGTTAAAACCAACACTACCACATGATCCA 426-485 Antisense
S13 TGCGTGTCTTTCTGCTATATGCATCATATGGAGCTTCCAACAGGAGTACACGCTGGTACT 466-525 Sense
S14 GTCTGTCAACAAATGGACCATAGAATTTACCTTCTAAGTCAGTACCAGCGTGTACTCCTG 506-565 Antisense
S15 TGGTCCATTTGTTGACAGACAAACTGCACAGGCTGCAGGTACAGACACAACCATAACATT 546-605 Sense
S16 ACCATTGATAACAGCAGCATACAGCCATGCCAAAACATTTAATGTTATGGTTGTGTCTGT 586-645 Antisense
S17 ATGCTGCTGTTATCAATGGTGATAGGTGGTTTCTTAATAGATTCACCACTACTTTGAATG 626-685 Sense
S18 AAAGGTTCATAGTTGTACTTCATTGCCACAAGGTTAAAGTCATTCAAAGTAGTGGTGAAT 666-725 Antisense
S19 AAGTACAACTATGAACCTTTGACACAAGATCATGTTGACATATTGGGACCTCTTTCTGCT 706-766 Sense
S20 TCAAAGCAGCACACATATCTAAGACGGCAATTCCTGTTTGAGCAGAAAGAGGTCCCAATA 746-805 Antisense
S21 AGATATGTGTGCTGCTTTGAAAGAGCTGCTGCAGAATGGTATGAATGGTCGTACTATCCT 786-845 Sense
S22 ATCAAATGGTGTAAACTCATCTTCTAAAATAGTGCTACCAAGGATAGTACGACCATTCAT 826-885 Antisense
S23 ATGAGTTTACACCATTTGATGTTGTTAGACAATGCTCTGGTGTTACCTTCCAAGGTAAGTTCAAGAAA 866-948 Sense
SProR GGGAGGGGGCTCGAGTCATTTCTTGAACTTACCTTGb 916-948 Antisense
a

Numerical position on the SCoV 3C-like protease.

b

Underlining indicates an XhoI restriction site.