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. 2017 Mar 7;8:277. doi: 10.3389/fmicb.2017.00277

Figure 2.

Figure 2

ComE impacts on the cps transcription. (A) Quantification of the cps2A mRNA by qRT-PCR in D39 and its mutants. The amount of the cps2A PCR product in each sample was quantified by its average mean cycle threshold value and normalized with that of the 16srRNA in the same reaction. (B) Impact of ComE on transcription activity of the cps promoter as assessed by a beta-galactosidase reporter. The results of representative experiments are presented as means of three replicates ± standard deviations. **P < 0.01; ***P < 0.0001; ns, no significant difference.