. 2017 Mar 7;8:277. doi: 10.3389/fmicb.2017.00277

Table 1.

Bacterial strains and plasmids used in this study.

Strain/plasmid	Relevant properties	Antibiotic concentrations	Source or references
STREPTOCOCCUS PNEUMONIAE
D39			NCTC
S. pneumoniae CPM8	Erm^R	0.25 μg/ml	Lee and Morrison, 1999
D39ΔcomE	Erm^R	0.25 μg/ml	This study
D39::comE^D58E	D39-P_czcD-gfp+-comE^D58E; Zn²⁺ -dependent production; Tet^R	2.5 μg/ml	This study
D39ΔcomE::comE^D58E	D39ΔcomE-P_czcD-gfp+-comE^D58E; n²⁺-dependent production; Tet^R, Erm^R	Tet^R: 2.5 μg/ml Erm^R: 0.25 μg/ml	This study
D39-pEVP3-cps-promoter	pEVP3, Chl^R	2.5 μg/ml	This study
D39ΔcomE-pEVP3-cps-promoter	pEVP3-cps-promoter; D39ΔcomE; Erm^R, Chl^R	Erm^R:0.25 μg/ml Chl^R: 2.5 μg/ml	This study
D39ΔcomE-pEVP3-cps-promoter::comE^D58E	D39ΔcomE-P_czcD-gfp+-comE^D58E; pEVP3-cps-promoter; D39ΔcomE; Erm^R, Chl^R; Tet^R	Erm^R:0.25 μg/ml Chl^R: 2.5 μg/ml Tet^R: 2.5 μg/ml	This study
D39-pAE03	pAE03; Erm^R	0.25 μg/ml	This study
D39-pAE03-cps-promoter	pAE03-cps-promoter; Erm^R	0.25 μg/ml	This study
D39ΔcomE-pAE03-cps-promoter	pAE03-cps-promoter; D39ΔcomE; ${Tet}_{2}^{R}$ ; Erm^R	${Tet}_{2}^{R}$ :0.3 μg/ml Erm^R:0.25 μg/ml	This study
ESCHERICHIA COLI
E. coli BL21(DE3)	Expression strain		Takara
E. coli DH5a	Cloning strain		Takara
PLASMIDS
pJWV25	Zn²⁺-dependent production; Tet^R	Tet^R:12.5 μg/ml(in E. coli)	Eberhardt et al., 2009
pEVP3	Chl^R	20 μg/ml(in E. coli)	Pestova and Morrison, 1998
pAE03	Erm^R	50 μg/ml(in E. coli)	Eberhardt et al., 2009
pET-28a(+)	Kana^R, (Expression vector)	50 μg/ml(in E. coli)	Takara
pJWV25::comE^D58E	pJWV25 derivative, carrying comE^D58E fusion; Tet^R	Tet^R: 2.5 μg/ml	This study
pEVP3-cps-promoter	pEVP3 derivative, carrying cps promoter fusion; Chl^R	20 μg/ml(in E. coli)	This study
pAE03-cps-promoter	pAE03 derivative, carrying cps promoter fusion; Erm^R	50 μg/ml(in E. coli)	This study

Erm^R, Erythromycin^R; Tet^R, Tetracyclines^R; Chl^R, Chloromycetin^R; Kana^R, Kanamycin^R.