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. 2017 Mar 7;7:63. doi: 10.3389/fcimb.2017.00063

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Copyright © 2017 Hoppe, Ünal, Thiem, Grimpe, Goldmann, Gaßler, Richter, Shevchuk and Steinert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Contribution of PilY1 domains to twitching and sliding motility. For (A) twitching and (B) sliding motility assays stationary phase liquid cultures of L. pneumophila Corby wild type (WT), the pilY1-negative mutant (ΔpilY1), the complemented pilY1 mutant strains expressing the wild type PilY1 (ΔpilY1 WTPilY1), the PilY1 lacking the C-terminal PilY1 domain (ΔPilYdom) and the PilY1 lacking vWFa domain (ΔvWFa) were spotted on BCYE agar plates and grown at 30°C for 14 days. (C) Images of twitching motility and sliding translocation are representatives of three independent experiments. The central raised ring represents the initial inoculum and the outer rings show the motility zone. Migration areas were calculated with Adobe Photoshop 7.0.1. (t-test ^*p ≤ 0.05, ^**p ≤ 0.01, ^***p ≤ 0.001).