FIGURE 3.

Effect of heat stress on expression of PIMT protein in S. Typhimurium. Wild type and Δpimt strains of S. Typhimurium were grown at 37 or 42°C for 12 h. Cells were then pelleted and induction of PIMT protein was analyzed by SDS-PAGE followed by Western blotting against anti-PIMT antibodies. The incubation temperatures and samples loaded in the lanes are depicted in figure itself. Lane M is protein molecular weight markers. PIMT band is marked by arrow.