Table 2.

Polymerase chain reaction (PCR) conditions used for amplification of different genes.

S. No.	Gene/purpose	Initial denaturation (temp/time)	Denaturation (temp/time)	Annealing (temp/time)	Extension (temp/time)	Final extension (temp/time)
1	pimt deletion	95°C/5 min	95°C/30 s	42°C/30 s	72°C/1 min	72°C/10 min
2	pimt test	95°C/30 s	95°C/30 s	60°C/30 s	72°C/90 s	72°C/10 min