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. 2017 Mar 7;11:66. doi: 10.3389/fncel.2017.00066

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Copyright © 2017 Jin, Yu, Chen, Lu, Ding, Xie and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Endogenous nNOS is required for neuronal differentiation of embryonic NSCs. The monolayer-cultured NSCs from nNOS^-/- and WT embryonic mice differentiated for 4 days and then were fixed for stain. (A) Statistical graph showing the ratio of GFAP⁺ astrocytes. (B) Representatives of GFAP-labeled cells. (C) Statistical graph showing the ratio of β-III-Tubulin⁺ neurons. (D) Representatives of β-III-Tubulin⁺ cells. Nuclei were counterstained with Hoechst 33258. Scale bars = 50 μm. Data are mean ± SEM (n = 3); ^∗p<0.05 as compared with WT. GFAP, glial fibrillary acidic protein; nNOS^-/-, gene knockout of neuronal nitric oxide synthase; WT, wild-type.