Figure 5.

Toxoplasma gondii does not promote host susceptibility mediated by Dectin-1. Wild-type (WT) and Dectin-1^−/− mice were infected with different strains, doses, and routes by T. gondii, in order to check for differences in survival analysis and parasite burden. Survival curves of (A) mice infected by intraperitoneal route with 1 × 10² tachyzoites of RH strain (10 mice/group); (B) mice infected by intraperitoneal route with 1 × 10⁴ tachyzoites of Pru strain (10 mice/group); (C) mice infected by intraperitoneal route with 1 × 10³ and (D) 1 × 10⁵ tachyzoites of Me49 strain (10 mice/group); (E) mice infected by oral route with 30 cysts of Me49 strain (10 mice/group). (F) Chronic phase parasite load of the central nervous system of mice after 30 days of infection with five cysts of Me49 strain (6 mice/group), analyzed by real-time PCR. Survival rates were compared using Kaplan–Meier survival analysis, through a long-rank Mantel–Cox test, but no statistically significance differences were observed. Results for quantitative polymerase chain reaction (qPCR) were expressed as mean ± SEM and are representative of three independent experiments. Statistical significance of the qPCR results was assessed by Student’s t-test.