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Representative cross sections of skeletal muscle tissue. Muscle tissue was stained with H&E in the sham group (A–D) and the icing group (F–I) at 1, 3, 7, and 28 d after injury. (E) Shows muscle tissue from an uninjured rat for comparison. Arrowheads indicate necrotic muscle fibers. Arrows indicate regenerating muscle fibers (centrally placed nuclei). Scale bar = 100 μm.
