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. 2017 Mar 7;8:299. doi: 10.3389/fpls.2017.00299

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Copyright © 2017 Zhao, Chang, Qi, Dong, Wang, Fan, Jiang, Cheng, Chen, Han, Xu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GmERF113 transcription activation assay. The GmERF113 transcription activation assay was performed in the Y2HGold yeast strain. (A) Schematic diagram of the expression and reporter constructs. The GmERF113 gene was fused in-frame to the GAL4 DNA-binding domain (BD) expression vector and pGADT7, and transformed into yeast strain Y2HGold. The yeast strain Y2HGold contained the ADE2 and HIS3 reporter genes under distinct GAL4-responsive promoter elements. (B) The full-length GmERF113 transcription activation assay. (C) GmERF113-I and GmERF113-II transcription activation assay. Yeast cells were selected by growth on SD (-Trp, -Leu) and (-Trp, -Leu, -His, -Ade) media. Yeast Y2HGold cells carrying pGBKT7-P53 and pGADT7-SV40 served as positive controls, whereas co-expression of pGBKT7-lam and pGADT7-SV40 was used as a negative control (Clontech, USA).