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. 2017 Mar 7;8:299. doi: 10.3389/fpls.2017.00299

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Copyright © 2017 Zhao, Chang, Qi, Dong, Wang, Fan, Jiang, Cheng, Chen, Han, Xu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction of GmERF113 with GmbHLH in yeast cells and planta. (A) GmERF113-II interacted with GmbHLH in yeast cells. The yeast cells were selected on SD (-Trp, -Leu) (DDO) medium and interactions were evaluated based on the ability of cells to grow on selective SD (-Trp, -Leu, -His, -Ade) (QDO) medium containing X-α-Gal (20 μg mL^-1) and aureobasidin A (125 μgmL^-1) for 5 days. Yeast Y2HGold cells carrying pGBKT7-P53 and pGADT7-SV40 served as positive controls, whereas co-expression of pGBKT7-lam and pGADT7-SV40 was used as a negative control (Clontech, USA). (B) BiFC assay of the interaction of GmERF113 with GmbHLH. GmERF113-YFP^N and GmbHLH-YFP^C were co-transfected into Arabidopsis protoplasts and observed using a confocal microscope. Bright-field, YFP fluorescence (yellow), chlorophyll autofluorescence (red), and combined images were visualized. Bars, 10 μm.