Figure 8.

AS101 decreases glomerular caspase-1 activity and glomerular macrophages. IL-1β secretion in vitro. Caspase-1 activity is very late antigen-4 (VLA-4) dependent. Isolated glomerular macrophages were treated with or without Staphylococcus aureus Cowan I (SAC) (5 μg/ml) and various concentrations of AS101 at 10⁶/ml for 24 h. IL-1β (A) and caspase-1 activity (B) were evaluated. ^#p < 0.01 increase vs. control, *p < 0.01 decrease vs. SAC. The one-way ANOVA was used 96-well plates were coated with VCAM-1 or fibronectin (FN) or BSA. Isolated glomerular macrophages were cultured with SAC (5 μg/ml) for 24 h. Cells were detached and cultured at 10⁴/well in triplicates in the presence or absence of various AS101 concentrations for 1 h. Non-attached cells were washed out and attached cells were quantitated by XTT (C). ^#p < 0.01 increase vs. BSA; *p < 0.05 decrease vs. AS101 zero (VCAM-1 or FN). The two-way ANOVA was used. Isolated glomerular macrophages were cultured as described in (B) in the presence or absence of AS101 (1 μg/ml), αVLA-4 (5 μg/ml), αVLA-5 (5 μg/ml), or their combinations, with or without SAC for 24 h. Caspase-1 activity was evaluated (D). ^#p < 0.01 increase vs. control; **p < 0.01 decrease vs. SAC; *p < 0.05 decrease vs. SAC; ^##p < 0.05 decrease vs. αVLA-5 without AS101. Data are presented as mean ± SE from four different experiments. The one-way ANOVA was used.