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Detection of transgenic Arabidopsis seedlings via over-expression of VaERD15 from Vitis amurensis. (A) PCR analysis of VaERD15 in T3 transgenic Arabidopsis seedlings. (B) Leaves of VaERD15 positive Arabidopsis plants tested by GUS staining, with WT Arabidopsis plants as controls. (C) Southern blotting was carried out on plants considered positive due to previous detection. M, marker; WT, wild type line; P, plasmid (positive control); Ln, various transgenic lines of VaERD15.
