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. 2017 Mar 7;8:297. doi: 10.3389/fpls.2017.00297

Table 1.

Primers used in this study.

Sr. no. Primer Sequence Amplicon (bp)
1 VaERD15-3′RACE 5′-GTTCGTTCCGTTGGCATATCGGACGGT-3′
VaERD15-5′RACE 5′-CTCAAACTACCAGGAAACCCTAACTCGC-3′
2 VaERD15-F(rt VaERD15 -F) 5′-ATGGCTATGGAGGTAATTTCACGTAC -3′ 423
VaERD15-R(rt VaERD15 -R) 5′- TTACCGCGGCTGCTGAATCG -3′.
3 VaERD15- XbalI-F 5′-GCTCTAGAATGGCTATGGAGGTAATTTCACG-3′ 439
VaERD15-Kpn I -R 5′-GGGGTACCCCGCGGCTGCTGAATC-3′
4 VaERD15-NcoI -F(Southern) 5′-CTAGccatgg ATGGCTATGGAGGTAATTTCACG-3′ 442
VaERD15-Bglll -R 5′-GGAagatetCTACGGCGGCTGCTGAATCG-3′
5 Actin1-F 5′-GATTCTGGTGATGGTGTGAGT-3′ 168
Actin1-R 5′-GACAATTTCCCGTTC AGC AGT-3′
6 AtGAPDH-F 5′-TTGGTGAC AAC AGGTC AAGC A-3′
AtGAPDH-R 5′-AAACTTGTCGCTCAATGCAATC-3′
7 rt AtERD15-F 5′-AACTTCGACTTGGTACCCTGAT-3′ 116
rt AtERD15 -R 5′-GAAGATCAGCTACATCGATATGA-3′