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Detection of SARS coronavirus replicase products by indirect immunofluorescent staining. Vero E6 cells were infected with 1,000 TCID50 of SARS coronavirus Urbani, fixed and permeabilized at 4, 8, 11, 16, 20, or 24 h postinfection, and stained with anti-R3 and fluorescein isothiocyanate-conjugated anti-rabbit immunoglobulin as described in Materials and Methods.
