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Maximum optical density produced (Ymax AU) from a variety of nitrite concentrations from the growth of P. denitrificans PD1222 at pH 7.0, 7.5, 8.0 and 8.5, in a defined minimal medium. 100 μl cultures in 96-well plates were agitated at 400 rpm and measured by optical density at 600 nm (OD600nm). Bars denote standard error of at least three independent biological replicates (n = ≥3).
